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Description

Almost every LC lab operates in reversed-phase mode, but only a minority of users are taking advantage of the full capability in this separation technique. Troubleshooting problems is easier if you understand the technique, and tremendous improvements in selectivity, efficiency, and throughput are possible with only modest modifications. This one-day course will provide an overview on the features that allow an RP application to succeed and answer the questions that most users have. Simple rules for improving your method can achieve dramatic results in reducing solvent usage and/or time, but only if you understand the rules and their limitations. Also, not every separation problem can be solved with a C18 column. If C18 does not work for you, we will show you what other options are available. All discussions make extensive use of real-world case studies.

Register for this Short Course

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Track(s)

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  Industry/Manufacturing

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Course Outline

* Review of Fundamental Relationships
How to speak “chromatographer”
* Reversed-Phase Operating Mode
Six critical operating variables
* Column Selectivity in Reversed-Phase Mode
Why are columns different and how we can measure those differences
* Improving Isocratic Methods
Do you want faster analysis, or less solvent usage, or both
• Practical Operating Tips
* Why C18 Doesn’t Work and Solutions for Each Situation
Too Much Retention - Four ways to make it elute faster
Too Little Retention - Five techniques to make it stay on the column longer
Poor Peak Shape/Selectivity - Thinking outside of the C18 box
* Final Review and Discussion

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Learning Objectives

Understand the critical operating variables in reversed-phase LC
Learn why not all columns produce the same separation
Understand the calculations for upgrading your isocratic methods
Know what to do when you have too much or too little retention with a C18 column

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Additional Info

Categories (Up to 5):
Enantiomeric Separations,Environmental Analysis,Food,Liquid Chromatography,Pharmaceutical Sciences

Proposal Number:
SC14531

Scientific Specialties:
Chromatography – Liquid

Target Audience:
Method development staff Supervisory staff Experienced analysts

Type of Industry:
Analytical Chemistry/Equipment/Instruments,Biotechnology/Biochem,Chemicals/Chemical Equipment,Contract Laboratory,Environmental/Air/Water/Wastewater,Food/Beverage/Consumer Products,Manufacturing,Pharmaceutical